This invention is related to the method of production of PDMS-I based antimicrobial solution with the steps of activation of polymethylcyloxane (PDMS) with sulfuric acid (101), diluting PDMS and sulfuric acid solution by isopropyl alcohol (IPA) (102), heating the solution (103), cooling down this solution and adding iodine into it (104) and after adding iodine, mixing the mixture in order to obtain a solution containing PDMS-I complex (105) as this solution forms a water and bacteria proof film layer on the surface it is applied in order to serve as a barrier between the surface and environment which eliminated the need for the surgeons to use gloves especially in surgery and exhibited very high antimicrobial activity thanks to its coordinated use of iodine with activated PDMS.