Biologic preparation for relieving pain in neck, shoulder, waist, and leg and having function of strengthening the muscles and vessels, comprising an agent for promoting blood circulation and removing obstruction in channels, a joint bio-lubricant, a tendon and muscle strengthening agent, a cell physiological function conditioning agent, and an essential cell growth and metabolism agent. The agent for promoting blood circulation and removing obstruction in channels comprising panax notoginsen extract, panax notoginsenosidum, VB12; the joint bio-lubricant comprises hyaluronic acid or/and hyaluronate, polyglutamic acid or/and polyglutamate; the tendon and muscle strengthening agent comprises collagen, collagen peptides, glutamine, inositol, calcium phosphate, and calcium gluconate; the cell physiological function conditioning agent comprises disodium ATP, cytochrome C, trehalose, allantoin, taurine, vitamins, and coenzymes; and the essential cell growth and metabolism agent comprises amino acids, sugars, and organic acids.