The present invention relates to a method and a composition for preparing a novel lipoxylosin analog or a leukotriene analog using two kinds of lipoxygenase and, Musse Musse (. Mus musculus) Mice derived from 8- mice and tiandenia tiandensis (Burkholderia thailandensis)-environmental friendly 15- lipoxygenase from, strain , New lipoxylosin analogues and leukotriene analogs not reported to date can be prepared with high productivity and high yield, thereby being useful in various industrial fields such as, medicine, foods and cosmetics.